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KMID : 0363020090390030331
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Journal of Korean Academy of Periodontology
2009 Volume.39 No. 3 p.331 ~ p.337
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Induction of IL-8 and reactive oxygen species in periodontal Ligament cells by Aggregatibacter actinomycetemcomitans
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Lee Yang-Sin
Yu Yoon-Jeong
Cha Jeong-Heon
Park Hong-Gyu
Kim Sung-Whan
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Abstract
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Purpose: Interleukin (IL)-8 is one of pro-inflammatory cytokines. Reactive oxygen species (ROS) are reduced metabolites of O2. Aggregatibacter actinomycetemcomitans is one of representative periodontopathogens. To investigate the role of A. actinomycetemcomitans in IL-8 expression of periodontal ligament (PDL) cells, we estimated the production of IL-8 and ROS in A. actinomycetemcomitans treated PDL cells.
Methods: The IL-8 production was determined by enzyme-linked immunosorbent assay. The ROS production was estimated using H2DCFDA and FACS.
Results: A. actinomycetemcomitans increased the production of IL-8 and ROS at 10, 100, and 500 multiplicity of infection. N-acetylcysteine, an antioxidant of ROS, down-regulated the production of IL-8 induced by A. actinomycetemcomitans.
Conclusions: These results suggest that A. actinomycetemcomitans induces IL-8 production and ROS may act as a mediator in this process.
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KEYWORD
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Aggregatibacter actinomycetemcomitans, interleukin-8, periodontal Ligament, reactive oxygen species
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